Part:BBa_K4455005
FMDV 2A Peptide Optimized for Chlamydomonas Reinhardtii
FMDV is a 2A peptide originating from Foot and Mouth Disease Virus. 2A peptides are short peptides, typically ranging from 18-22 amino acids in length, which facilitate the expression of more than one protein in a single vector.
In viruses, 2A peptides enable the expression of multiple proteins from a single transcript. During translation, ribosomes which encounter 2A peptides will undergo a "ribosomal skipping event," in which a peptide bond fails to be made. This causes a "cleavage" event to take place between the two polyproteins between the 2A peptide sequence, creating two independent polyproteins.
The sequences for 2A peptides found in viruses, like FMDV, can be used in synthetic biology to allow the efficient coexpression of genes in novel biological entities, as they do in their native context. In addition, the small size and abundance of 2A peptide sequences available add to their utility in gene editing.
This part is from the Chlamydomonas MoClo Toolkit, which is a standardized engineering toolkit based on Golden Gate cloning for use in Chlamydomonas reinhardtii. In the MoClo Toolkit, this composite part is a level 0 plasmid called pCM0-087. In this level 0 plasmid, the FMDV 2A peptide sequence is flanked by standardized golden gate restriction enzyme sites for assembly of the part into level 1 modules. This level 0 plasmid also contains a spectinomycin resistance cassette for use in E.coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 19
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 859
- 1000COMPATIBLE WITH RFC[1000]
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